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- Title
Engineered Serratia marcescens for efficient (3 R)-acetoin and (2 R,3 R)-2,3-butanediol production.
- Authors
Bai, Fangmin; Dai, Lu; Fan, Jiying; Truong, Ngoctu; Rao, Ben; Zhang, Liaoyuan; Shen, Yaling
- Abstract
(3 R)-Acetoin and (2 R,3 R)-2,3-butanediol are important pharmaceutical intermediates. However, until now, the quantity of natural microorganisms with the ability to produce single configuration of optically pure (3 R)-acetoin and (2 R,3 R)-2,3-butanediol is rare. In this study, a meso-2,3-butanediol dehydrogenase encoded by the slaC gene from Serratia marcescens MG1 was identified for meso-2,3-butanediol and (2 S,3 S)-2,3-butanediol biosynthesis. Inactivation of the slaC gene could significantly decrease meso-2,3-butanediol and (2 S,3 S)-2,3-butanediol and result in a large quantity of (3 R)-acetoin accumulation. Furthermore, a (2 R,3 R)-2,3-butanediol dehydrogenase encoded by the bdhA gene from Bacillus subtilis 168 was introduced into the slaC mutant strain of Serratia marcescens MG1. Excess (2 R,3 R)-2,3-butanediol dehydrogenase could accelerate the reaction from (3 R)-acetoin to (2 R,3 R)-2,3-butanediol and lead to (2 R,3 R)-2,3-butanediol accumulation. In fed-batch fermentation, the excess (2 R,3 R)-2,3-butanediol dehydrogenase expression strain could produce 89.81 g/l (2 R,3 R)-2,3-butanediol with a productivity of 1.91 g/l/h at 48 h. These results provided potential applications for (3 R)-acetoin and (2 R,3 R)-2,3-butanediol production.
- Subjects
BUTANEDIOL manufacturing; SERRATIA marcescens; ACETOIN; DEHYDROGENASES; BIOACCUMULATION; BACILLUS subtilis genetics; BIOTECHNOLOGICAL microorganisms
- Publication
Journal of Industrial Microbiology & Biotechnology, 2015, Vol 42, Issue 5, p779
- ISSN
1367-5435
- Publication type
Article
- DOI
10.1007/s10295-015-1598-5