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- Title
改良石蜡切片法观察金针菇子实体不同发育时期组织结构.
- Authors
王瑞娟; 万佳宁; 龚明; 茅文俊; 汪莹; 唐利华; 尚俊军; 杨慧; 鲍大鹏
- Abstract
The improved paraffin method was used to prepare sections of Flammulina filiformis fruiting bodies at different developmental stages, and to observe cell structure of gills and stipes. The results showed that F. filiformis fruiting body section was clear, intact and wrinkle-free with limonene as clearing agent. Observation of tissue sections of F. filiformis fruiting bodies showed that there was a pileus structure with irregularly twisted mycelium in primordium. With the development of fruiting body, the gills differentiated in the bottom of pileus; after that, the stipe structure was further differentiated with the development of pileus. The stipe cells were longitudinally closely aligned in F. filiformis. Viewing from the cross section, vascular bundle cells were arranged as compacting inside and loosening outside with cavities in it. A heteromorphic structure was observed, where mycelia and bulliform cells coexisted in gill trama.
- Subjects
FRUIT development; CELL anatomy; PARAFFIN wax; GILLS; MYCELIUM
- Publication
Acta Edulis Fungi, 2019, Vol 26, Issue 2, p54
- ISSN
1005-9873
- Publication type
Article
- DOI
10.16488/j.cnki.1005-9873.2019.02.008