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- Title
高效液相色谱法测定川贝母和川贝粉中 三嗪代谢物残留.
- Authors
郭新颖; 陈 峰
- Abstract
Objective To set up a rapid solid phase extraction combined high performance liquid chromatography (HPLC) method and the measuring unibract fritillary bulb of fritillary bulb powder in three triazine metabolites residues analysis methods. Methods The samples were extracted by acetonitrile-water, separated by inorganic salts such as anhydrous magnesium sulfate and sodium acetate, purified by octyl-bonded silica gel C18 Athena C18 column was used as the chromatographic column, water and acetonitrile were used as the mobile phase for gradient elution and quantitative determination by external standard method. Results By selecting the appropriate chromatographic column, mobile phase, and purification agent, under the optimal chromatographic conditions, the metabolism of three triazine metabolites, namely isopropylatrazine, diethylatrazine, and diethylterbutazine, the limits of detection and quantitation of the 3 kinds of triazine metabolites were 0.20 μg/kg and 0.67 μg/kg respectively. The linear relationship of the three target components was good (r≥0.999) in the linear range of 0.01–20.00 μg/mL. The average recoveries were 95.3%~105.7% and relative standard deviation were 1.0%–2.4% in the addition levels of 5.0、25 and 50 μg/kg. Conclusion The method is simple, rapid and sensitive, and is suitable for the rapid detection and analysis of triazine pesticide metabolites in the traditional Chinese medicinal materials.
- Publication
Journal of Food Safety & Quality, 2024, Vol 15, Issue 11, p189
- ISSN
2095-0381
- Publication type
Article
- DOI
10.19812/j.cnki.jfsq11-5956/ts.20240320005