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- Title
CD5 Deficiency Alters Helper T Cell Metabolic Function and Shifts the Systemic Metabolome.
- Authors
Whitley, Kiara V.; Freitas, Claudia M. Tellez; Moreno, Carlos; Haynie, Christopher; Bennett, Joshua; Hancock, John C.; Cox, Tyler D.; Pickett, Brett E.; Weber, K. Scott
- Abstract
Metabolic function plays a key role in immune cell activation, destruction of foreign pathogens, and memory cell generation. As T cells are activated, their metabolic profile is significantly changed due to signaling cascades mediated by the T cell receptor (TCR) and co-receptors found on their surface. CD5 is a T cell co-receptor that regulates thymocyte selection and peripheral T cell activation. The removal of CD5 enhances T cell activation and proliferation, but how this is accomplished is not well understood. We examined how CD5 specifically affects CD4+ T cell metabolic function and systemic metabolome by analyzing serum and T cell metabolites from CD5WT and CD5KO mice. We found that CD5 removal depletes certain serum metabolites, and CD5KO T cells have higher levels of several metabolites. Transcriptomic analysis identified several upregulated metabolic genes in CD5KO T cells. Bioinformatic analysis identified glycolysis and the TCA cycle as metabolic pathways promoted by CD5 removal. Functional metabolic analysis demonstrated that CD5KO T cells have higher oxygen consumption rates (OCR) and higher extracellular acidification rates (ECAR). Together, these findings suggest that the loss of CD5 is linked to CD4+ T cell metabolism changes in metabolic gene expression and metabolite concentration.
- Subjects
T helper cells; CELL physiology; T cell receptors; T cells; CELL metabolism
- Publication
Biomedicines, 2022, Vol 10, Issue 3, p704
- ISSN
2227-9059
- Publication type
Article
- DOI
10.3390/biomedicines10030704