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- Title
半夏泻心汤加减方治疗多囊卵巢综合征合并胰岛素抵抗的作用及机制.
- Authors
贾凡; 赵红玉; 郑冬雪; 陈如枫; 陈彤; 郁悦; 张娜娜; 朱诗琴; 张武凡; 王超颖; 赵一鸣; 刘新敏
- Abstract
Objective We aimed to explore the therapeutic effect of Modified Banxia Xiexin Decoction (BXXX) in the treatment of polycystic ovary syndrome ( PCOS) complicated with insulin resistance (IR) through regulating the PI3K/ Akt / GLUT4 signaling pathway. Methods Letrozole combined with a high-fat diet was used to construct a PCOS and IR rat model. Model rats were divided into 6 groups, namely the control group, the model group, the metformin group[0. 157 5 g / (kg·d)], and the BXXX high-, medium-, low-dose groups[26. 46、13. 23、6. 615 g ( kg·d )], with 6 rats per group, and the corresponding medication was given by gavage for 2 weeks. 6 normal rats were taken as the control group. Fasting blood glucose(FBG) levels were measured. Pathological changes in ovarian tissues were observed via HE staining. Serum sex hormone levels, glucose levels, and lipid metabolism indexes were measured by ELISA. The expression levels of PI3K/ Akt / GLUT4 signaling pathway-related factors in ovarian tissues of PCOS and IR rats were determined by RT-PCR, IHC staining, and Western blotting. Results Compared with the control group, the structure of the follicles in the model group was abnormal, the number of granular cell layers was reduced, and more cystic dilated follicles were observed. T levels, HOMA-IR levels, and TC and TG levels were increased (P<0. 05); and the mRNA levels of Akt1 and the protein expression levels of PI3K, p-PI3K, Akt1, p-Akt1, and GLUT4 were decreased (P<0. 05). The corpus luteum and follicles were observed at different developmental stages. Compared with the model group, in the metformin group, BXXX high-dose group, BXXX medium-dose group, and BXXX low-dose group, the number of granulosa cell layers was increased and the number of cystic dilated follicles was decreased or even zero. The T levels in the metformin group and BXXX highdose group and the TC and TG levels in BXXX high-dose group were decreased (P<0. 05); and the mRNA levels of PI3K and Akt1 in the metformin group, BXXX high-, medium-, low-dose group were increased (P > 0. 05). The GLUT4 mRNA level in the metformin group, BXXX high-, medium-dose group was increased (P > 0. 05). The expression levels of p-PI3K and GLUT4 in the metformin group, BXXX high-, medium-, low-dose group were significantly increased (P<0. 05). Conclusion Modified Banxia Xiexin Decoction can improve IR and reproductive endocrine levels in PCOS and IR rats to treat PCOS. Its mechanism is related to the regulation of PI3K/ Akt / GLUT4 signaling pathway-related factors.
- Subjects
POLYCYSTIC ovary syndrome; CORPUS luteum; GRANULOSA cells; HIGH-fat diet; PATHOLOGICAL physiology
- Publication
Journal of Beijing University of Traditional Chinese Medicine, 2023, Vol 46, Issue 8, p1117
- ISSN
1006-2157
- Publication type
Article
- DOI
10.3969/j.issn.1006-2157.2023.08.010