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- Title
Temporal and tissue localization of a cowpea (Vigna unguiculata) cystatin.
- Authors
Flores, Victor M. Q.; Louro, Ricardo P.; Xavier-Filho, José; Barratt, Daniel H. P.; Shewry, Peter R.; Fernandes, Kátia V. S.
- Abstract
A cowpea (Vigna unguiculata L. Walpers cv. Pitiúba) cystatin was analysed to determine its localization during development and germination of cowpea seeds, using western blotting with a specific antiserum. The pattern of immunoreactive proteins changed during development, with the major reactive bands present in dried seeds being mobilized after a 62-h period of imbibition. Immunohistochemical analysis revealed that cowpea cystatin is distributed in both embryonic axes and cotyledons with the highest level being present in the outer cell walls of the adaxial surface of the cotyledons.
- Subjects
COWPEA; GERMINATION
- Publication
Physiologia Plantarum, 2001, Vol 112, Issue 2, p195
- ISSN
0031-9317
- Publication type
Article
- DOI
10.1034/j.1399-3054.2001.1120207.x