We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Modulation of muramyl dipeptide stimulation of cytokine production by blood components.
- Authors
van der Meer, J. H. M.; Netea, M. G.; Dinarello, C. A.
- Abstract
Muramyl dipeptide (MDP) is the minimal active fragment of peptidoglycan of the cell wall of Gram-positive bacteria, with potential beneficial effects as a vaccine adjuvant. Peptidoglycans and MDP are recognized by the intracellular receptor NOD2 (nucleotide-binding oligomerization domain 2), leading to production of proinflammatory cytokines. In the present study, it is shown that, despite stimulatory effects on isolated human mononuclear cells, MDP does not stimulate production of tumour necrosis factor-α, interleukin-1β or interleukin-6 in a whole-blood assay. However, MDP retains synergistic effects on lipopolysaccharide-induced cytokines in whole blood. Screening tests of NOD2 function based on whole-blood stimulation should therefore employ strategies based on the synergistic effects of MDP on Toll-like receptor-induced cytokine production. Plasma was not responsible for the inhibition of MDP in whole blood. The inhibition of MDP stimulation was dependent upon cellular components, with erythrocyte-derived haemoglobin and neutrophils collaborating in the inhibition of MDP effects in whole blood.
- Subjects
CYTOKINES; HEMOGLOBINS; NEUTROPHILS; MONONUCLEOSIS; INTERLEUKIN-6; PEPTIDOGLYCANS; ERYTHROCYTES
- Publication
Clinical & Experimental Immunology, 2009, Vol 156, Issue 3, p428
- ISSN
0009-9104
- Publication type
Article
- DOI
10.1111/j.1365-2249.2009.03926.x