We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Partial CD8+T-cell depletion of allogeneic peripheral blood stem cell transplantation is insufficient to prevent graft-versus-host disease.
- Authors
Ho, V. T.; Kim, H. T.; Li, S.; Hochberg, E. P.; Cutler, C.; Lee, S. J.; Fisher, D. C.; Milford, E.; Kao, G.; Daley, H.; Levin, J.; Ng, A.; Mauch, P.; Alyea, E. P.; Antin, J. H.; Soiffer, R. J.
- Abstract
Summary:Prior studies suggest that depletion of CD8+T cells from donor bone marrow or donor lymphocyte infusions can reduce graft-versus-host disease (GVHD) without compromising graft-versus-leukemia. We explored CD8 depletion in patients undergoing matched related donor (MRD, n=25) and unrelated donor (URD, n=16) peripheral blood stem cell transplantation following myeloablative conditioning with cyclophosphamide (60?mg/kg/day i.v.×2) and total body irradiation (200?cGy×7 fractions). Ex vivo incubation of mobilized donor peripheral blood cells with anti-CD8 antibody coated high-density microparticles removed 99%of CD8+cells. The median number of CD8+cells infused was 3.9×105 cells/kg (2.2×105 in MRD, and 8.1×105 in URD patients). Post transplant immune suppression included tacrolimus in the MRD cohort, and tacrolimus plus mini-methotrexate (5?mg/m2 days+1, 3, 6, 11) in the URD cohort. All 41 patients engrafted. Grade 2-4 acute GVHD incidence was 61%(44%MRD, 88%URD). Chronic GVHD incidence was 50%(48%MRD, 55%URD). Relapse incidence was 4.9%. Estimated event-free and overall survival rates were 65 and 63%, respectively, at 1 year and 56 and 57%, respectively, at 2 years. There was no correlation between CD8+number and GVHD or survival. A 2-log depletion of CD8+cells from PBSC is insufficient to prevent GVHD.Bone Marrow Transplantation (2004) 34, 987-994. doi:10.1038/sj.bmt.1704690 Published online 18 October 2004
- Subjects
STEM cell transplantation; BONE marrow transplantation; BONE marrow; HOMOGRAFTS; LYMPHOCYTE transformation; TRANSPLANTATION of organs, tissues, etc.
- Publication
Bone Marrow Transplantation, 2004, Vol 34, Issue 11, p987
- ISSN
0268-3369
- Publication type
Article
- DOI
10.1038/sj.bmt.1704690