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- Title
SBD.4 stimulates regenerative processes in vitro, and wound healing in genetically diabetic mice and in human skin/severe-combined immunodeficiency mouse chimera.
- Authors
Zhao, Hui; Mortezaei, Reza; Wang, Yanrong; Sheng, Xinsheng; Aria, Fariba; Bojanowski, Krzysztof
- Abstract
In search of novel angiostimulators, we performed a high-throughput screening of medicinal plants beneficial for blood circulation. From the panel of positive hits, Angelica sinensis was selected for further investigation. Purified down to a low-molecular-weight fraction and characterized by high-performance liquid chromatography–mass spectrometry, the material, named SBD.4A, revealed a particularly strong wound healing activity in the diabetic mouse wound-healing model, and in the human/severe combined immunodeficiency mouse chimera wound-healing model. In both models, SBD.4A compared favorably with the Food and Drug Administration-approved wound-healing drug becaplermin, suggesting that this botanical product could be a valuable treatment for difficult-to-heal wounds. Further high-performance liquid chromatography fractionation of SBD.4A yielded a hydrophilic fraction, which strongly stimulated endothelial cell proliferation, tridimensional endothelial cell network formation, as well as the proliferation of human dermal fibroblasts and type I collagen secretion. Because angiogenesis and fibroblast proliferation are essential for wound healing, we propose that this liquid chromatography–mass spectrometry-defined hydrophilic fraction is at least partially responsible for the wound-healing activity of SBD.4A.
- Subjects
WOUND healing; REGENERATION (Biology); LIQUID chromatography; MASS spectrometry; HEALING
- Publication
Wound Repair & Regeneration, 2006, Vol 14, Issue 5, p593
- ISSN
1067-1927
- Publication type
Article
- DOI
10.1111/j.1743-6109.2006.00166.x