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- Title
A Glycosyltransferase from Arabidopsis thaliana Enables the Efficient Enzymatic Synthesis of Gastrodin.
- Authors
Xia, Hui; Zhang, Zihan; Ding, Ji; Jiang, Kehan; Xue, Feng
- Abstract
Gastrodin is an important plant-derived medicinal ingredient that has been used to treat various diseases. Biosynthesis is an effective strategy for gastrodin production, but the expression and activity of natural glycosyltransferases (GTs) is generally a limiting factor. To identify highly efficient GTs, four plant-derived enzymes were identified by homologous sequence analysis and expressed in Escherichia coli BL21 (DE3). A GT from Arabidopsis thaliana (AtUGT) displayed the highest activity of 0.45 U/mg, with an optimal pH of 9.0 and high thermostability. Under optimized conditions, 10 mM p-hydroxybenzyl alcohol and uridine diphosphate glucose were converted into 2.67 g/L gastrodin, corresponding to a conversion rate of 94.34% in 12 h. Furthermore, molecular docking simulations were performed between AtUGT and p-hydroxybenzyl alcohol, revealing the catalytic mechanism of AtUGT. This study provides a novel and effective biocatalyst for the synthesis of gastrodin.
- Subjects
URIDINE diphosphate; MOLECULAR docking; SEQUENCE analysis; ESCHERICHIA coli; GLYCOSYLTRANSFERASES
- Publication
Catalysis Letters, 2024, Vol 154, Issue 4, p1558
- ISSN
1011-372X
- Publication type
Article
- DOI
10.1007/s10562-023-04406-y