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- Title
Evaluation of Putative Inducers and Inhibitors toward Tyrosinase from two Trichoderma species.
- Authors
El-Shora, Hamed M.; El-Sharkawy, Reyad M.
- Abstract
The present investigation deals with the purification of extracellular tyrosinase produced by Trichoderma reesei and Trichoderma harzianum and the characterization of putative inducers and inhibitors on the enzyme activity. Tyrosinase enzyme from T. reesei and T. harzianum was purified to homogeneity using ammonium sulphate precipitation (85%) followed by DEAE-cellulose, and phenyl Sepharose. SDS-PAGE exhibited a single band of 45 and 65 kDa for the purified tyrosinase from T. reesei and T. harzianum, respectively. The influence of guaiacol, catechol, vanillin, caffeic acid, syringaldazine and p-coumaric acid on purified enzyme was tested. Tyrosinase produced by the two Trichoderma species displayed a dose-dependent response toward all tested inducers. The effect of kojic acid, cinnamic acid, sodium azide, benzaldehyde and potassium cyanide was assayed at pH 5.0 in presence of monophenolic substrate (p-coumaric acid) and diphenolic substrate (caffeic acid). KCN and kojic acid were the powerful inhibitors on the tyrosinase-monophenolase and diphenolase activities from the investigated Trichoderma, respectively. The rate of tyrosinase activity showed a pseudo-firstorder reaction kinetics and was proportional to the inhibitor concentration. The results reveal the possibility of using the inducers of tyrosinase to increase its activity.
- Subjects
HYDROXYCINNAMIC acids; POTASSIUM cyanide; TRICHODERMA; TRICHODERMA reesei; CAFFEIC acid; CINNAMIC acid
- Publication
Jordan Journal of Biological Sciences, 2020, Vol 13, Issue 1, p7
- ISSN
1995-6673
- Publication type
Article