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- Title
Secondary immunisation with high-dose heterologous peptide leads to CD8 T cell populations with reduced functional avidity.
- Authors
Narayan, Sharmal; Choyce, Allison; Fernando, Germain J. P.; Leggatt, Graham R.
- Abstract
Expansion of high- or low-avidity CD8 T cells in vitro inversely correlates with the concentration of peptide ligand present during culture. In contrast, the selective enrichment of high- or low-avidity T cell populations in vivo using peptide immunisation is not well documented. In our study, a single immunisation with different doses of wild-type peptide or a variant peptide able to stimulate CTL responses cross-reactive with wild-type peptide failed to shift the average avidity of the responding CD8 T cell population specific to either peptide. However, in contrast to homologous prime-boost immunisation, heterologous prime-boost immunisation incorporating high doses of the second immunogen resulted in peptide-specific CD8 T cell populations polarized toward a low average functional avidity. These data suggest that sequential exposure to structurally related viral peptides could impair rather than promote anti-viral immunity by lowering the avidity of the responding CD8 T cell population. This study has implications for improving vaccine strategies against viruses and tumours and enhances our understanding of heterologous immunity during sequential viral infection.
- Publication
European Journal of Immunology, 2007, Vol 37, Issue 2, p406
- ISSN
0014-2980
- Publication type
Article
- DOI
10.1002/eji.200535688