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- Title
Pituitary adenylate cyclase-activating polypeptide inhibits cutaneous immune function.
- Authors
Kodali, Sreedevi; Friedman, Ilyse; Ding, Wanhong; Seiffert, Kristina; Wagner, John A.; Granstein, Richard D.
- Abstract
Epidermal nerves are closely associated with Langerhans cells (LC) and may be able to release factors, such as calcitonin gene-related peptide and epinephrine, that affect LC function. LC and the LC-like cell line XS106 express mRNA for the pituitary adenylate cyclase-activating polypeptide (PACAP) receptors VPAC1 and VPAC2. We examined whether PACAP regulates cutaneous immunity. Intradermal administration of PACAP prior to application of a contact sensitizer at the injected site inhibited the induction of contact hypersensitivity. Pretreatment of murine epidermal cells enriched for LC content (∼12% LC) with PACAP inhibited their ability to elicit delayed-type hypersensitivity in previously immunized mice. In vitro, PACAP suppressed the ability of both murine epidermal cells and highly purified LC (∼95%) to present antigen to a T cell clone and hybridoma. Furthermore, in LC and the XS106 cell line, PACAP inhibited the LPS/GM-CSF-induced stimulation of IL-1β secretion and augmented IL-10 production. PACAP also down-regulated CD86 expression in LPS/GM-CSF-stimulated XS106 cells. The immunosuppressive effects of PACAP may be due to modulation ofcytokine production and CD86 expression.
- Publication
European Journal of Immunology, 2003, Vol 33, Issue 11, p3070
- ISSN
0014-2980
- Publication type
Article
- DOI
10.1002/eji.200324085