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- Title
Effect of Fetal Bovine Serum Concentration on Lysophosphatidylcholine-mediated Proliferation and Apoptosis of Human Aortic Smooth Muscle Cells.
- Authors
Daisuke Asai; Takahito Kawano; Masaharu Murata; Hideki Nakashima; Riki Toita; Jeong-Hun Kang
- Abstract
Lysophosphatidylcholine (lysoPtdCho) is produced by the phospholipase A2-mediated hydrolysis of phosphatidylcholine and can stimulate proliferation and apoptosis of vascular smooth muscle cells. We examined the influence of fetal bovine serum (FBS) concentration in the culture medium on lysoPtdChomediated apoptosis and proliferation of human aortic smooth muscle cells (HASMCs) as well as on the activation of extracellular signal-regulated kinases (ERK)1/2. In the presence of 1% FBS, HASMC viability increased after lysoPtdCho treatment at 1 and 10 μM but decreased at 25 and 50 μM. However, lysoPtdCho increased HASMC viability in a dose-dependent manner in the presence of 10% FBS. The activity of caspase 3/7 in HASMCs was increased by 25 μM lysoPtdCho in the presence of 1% FBS, but not 10% FBS. Furthermore, lysoPtdCho at 1 and 10 μM triggered ERK1/2 phosphorylation in the presence of 1% FBS, but not at 10% FBS. Thus, lysoPtdCho-mediated HASMC apoptosis, proliferation, and ERK1/2 activation are dependent on the concentration of FBS.
- Subjects
LYSOPHOSPHATIDYLCHOLINE acyltransferase; PHOSPHOLIPASE A2; APOPTOSIS; VASCULAR smooth muscle; LECITHIN
- Publication
Journal of Oleo Science, 2020, Vol 69, Issue 3, p255
- ISSN
1345-8957
- Publication type
Article
- DOI
10.5650/jos.ess19268