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- Title
Direct plasma stimuli including electrostimulation and OH radical induce transient increase in intracellular Ca<sup>2+</sup> and uptake of a middle‐size membrane‐impermeable molecule.
- Authors
Sasaki, Shota; Hokari, Yutaro; Kumada, Akiko; Kanzaki, Makoto; Kaneko, Toshiro
- Abstract
Despite the promising potential of medical treatments based on non‐equilibrium atmospheric‐pressure plasma, most of the underlying mechanisms remain unknown. Here we report that direct plasma irradiation (DPI) can induce transient increase in intracellular calcium (Ca2+) concentrations, thereby playing key roles in many cellular processes as well as the uptake of middle‐size membrane‐impermeable molecules, which did not occur with indirect plasma irradiation (IPI). Experimental measurements using a Pockels voltage probe and gelling chemical reagent showed that electric field of 150 kV/cm and hydroxyl radical (OH) supply to cell cultures were induced by DPI under a typical condition compared with IPI. In addition, significant inhibition of the OH scavenger D‐mannitol indicated involvement of OH in those DPI‐induced cellular responses. These results facilitate the elucidation of plasma‐cell interaction and the development of plasma devices for medical treatments.
- Subjects
HYDROXIDES; REACTION mechanisms (Chemistry); INTRACELLULAR calcium; ELECTRIC fields; HYDROXYL group
- Publication
Plasma Processes & Polymers, 2018, Vol 15, Issue 1, p1
- ISSN
1612-8850
- Publication type
Article
- DOI
10.1002/ppap.201700077