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- Title
The effect of krill oil on Wnt/β-catenin signaling pathway in acetaminophen-induced acute liver injury in mice.
- Authors
Şahin, Yaşar; Devrim, Alparslan Kadir; Alçığır, Mehmet Eray; Şenol, Ali; Ekici, Hüsamettin; Devrim, Tuba; Sudağıdan, Mert; Yıldırım, Ebru; Çınar, Miyase; Türkmen, Merve Bişkin; Peker, Seydi Ali
- Abstract
This study investigated the effect of krill oil (KO) on liver damage caused by acetaminophen (APAP). In the present study, the control and APAP groups were given distilled water by gavage for 14 days. In addition, the KO and APAP+KO groups were given 500 mg/kg krill oil by gavage for 14 days. At the end of 14 days, 0.9 % sodium chloride solution (saline solution) administration was applied intraperitoneally to the control and KO groups. Meanwhile, 220 mg/kg acetaminophen was administered to the APAP and APAP+KO groups. While some biochemical parameters in plasma were examined, some oxidative stress parameters in plasma and liver tissue were evaluated. Apoptotic and inflammatory responses of some primer sequences determined by quantitative Real-Time PCR (qPCR) in liver tissue. After histopathological examination of liver tissue, immunohistochemical analysis was performed with Wnt inhibitory factor-1 (Wif-1), beta-catenin (β-Catenin), and 8-hydroxy-2′-deoxyguanosine (8-OHdG). The Wif-1 positivity in hepatocytes increased significantly in the APAP group (5.29 ± 0.71) compared to the control (1.14 ± 0.51), and KO (2.14 ± 0.55) groups (p<0.001). The 8-OHdG positivity in hepatocytes increased significantly in the APAP group (19.57 ± 0.58) compared to the control (0.43 ± 0.20), KO (3.57 ± 0.48), and APAP+KO (4.00 ± 2.53) groups (p<0.001). As a result, krill oil could be used as a nutritional supplement to protect the liver against acetaminophen-induced liver injury.
- Subjects
KRILL oil; LIVER injuries; CELLULAR signal transduction; SALINE solutions; DIETARY supplements; LIVER
- Publication
Turkish Journal of Biochemistry / Turk Biyokimya Dergisi, 2023, Vol 48, Issue 3, p264
- ISSN
0250-4685
- Publication type
Article
- DOI
10.1515/tjb-2022-0289