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- Title
A framework for gene mapping in wheat demonstrated using the Yr7 yellow rust resistance gene.
- Authors
Gardiner, Laura-Jayne; Bansept-Basler, Pauline; El-Soda, Mohamed; Hall, Anthony; O'Sullivan, Donal M.
- Abstract
We used three approaches to map the yellow rust resistance gene Yr7 and identify associated SNPs in wheat. First, we used a traditional QTL mapping approach using a double haploid (DH) population and mapped Yr7 to a low-recombination region of chromosome 2B. To fine map the QTL, we then used an association mapping panel. Both populations were SNP array genotyped allowing alignment of QTL and genome-wide association scans based on common segregating SNPs. Analysis of the association panel spanning the QTL interval, narrowed the interval down to a single haplotype block. Finally, we used mapping-by-sequencing of resistant and susceptible DH bulks to identify a candidate gene in the interval showing high homology to a previously suggested Yr7 candidate and to populate the Yr7 interval with a higher density of polymorphisms. We highlight the power of combining mapping-by-sequencing, delivering a complete list of gene-based segregating polymorphisms in the interval with the high recombination, low LD precision of the association mapping panel. Our mapping-by-sequencing methodology is applicable to any trait and our results validate the approach in wheat, where with a near complete reference genome sequence, we are able to define a small interval containing the causative gene.
- Subjects
STRIPE rust; GENE mapping; WHEAT; GENES; PANEL analysis; WHEAT diseases &; pests; GENETIC recombination
- Publication
PLoS ONE, 2020, Vol 15, Issue 4, p1
- ISSN
1932-6203
- Publication type
Article
- DOI
10.1371/journal.pone.0231157