We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Leukocyte and cytokine variables in asymptomatic Pugs at genetic risk of necrotizing meningoencephalitis.
- Authors
Windsor, Rebecca; Stewart, Samuel D.; Talboom, Joshua; Lewis, Candace; Naymik, Marcus; Piras, Ignazio S.; Keller, Stefan; Borjesson, Dori L.; Clark, Gary; Khanna, Chand; Huentelman, Matthew
- Abstract
Background: Necrotizing meningoencephalitis (NME, aka Pug dog encephalitis) is an inflammatory brain condition associated with advanced disease at initial presentation, rapid progression, and poor response to conventional immunomodulatory therapy. Hypothesis/Objectives: That genetic risk for NME, defined by a common germline DNA haplotype located on chromosome 12, is associated with altered blood cytokine concentrations and leukocyte subsets in asymptomatic Pugs. Animals Forty Pug dogs asymptomatic for NME from a hospital sample. Methods: Prospective observational cohort study, including germline genome‐wide genotyping, plasma cytokine determination by multiplexed profiling, and leukocyte subset characterization by flow cytometric analysis. Results: Seven (18%) dogs were high risk, 10 (25%) medium risk, and 23 (58%) low risk for NME, giving a risk haplotype frequency of 30%. High and medium risk Pugs had significantly lower proportion of CD4+ T cells (median 22% [range, 7.3%‐38%] vs 29% [range, 16%‐41%], P =.03) and higher plasma IL‐10 concentrations than low‐risk Pugs (median 14.11 pg/mL [range, 9.66‐344.19 pg/mL] vs 12.21 pg/mL [range, 2.59‐18.53 pg/mL], P =.001). No other variables were significantly associated with the NME haplotype‐based risk. Conclusions and Clinical Importance: These data suggest an immunological underpinning to NME and a biologic rationale for future clinical trials that investigate novel diagnostic, preventative, and therapeutic strategies for this disease.
- Subjects
MENINGOENCEPHALITIS; LEUCOCYTES; CYTOKINES; T cells; SYMPTOMS
- Publication
Journal of Veterinary Internal Medicine, 2021, Vol 35, Issue 6, p2846
- ISSN
0891-6640
- Publication type
Article
- DOI
10.1111/jvim.16293