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- Title
Plasma miR-26a as a Diagnostic Biomarker Regulates Cytokine Expression in Systemic Juvenile Idiopathic Arthritis.
- Authors
Juan Sun; Miao Feng; Fengqi Wu; Xiaolin Ma; Jie Lu; Min Kang; Zhewei Liu; Sun, Juan; Feng, Miao; Wu, Fengqi; Ma, Xiaolin; Lu, Jie; Kang, Min; Liu, Zhewei
- Abstract
<bold>Objective: </bold>We sought to identify specific microRNA (miRNA) for systemic juvenile idiopathic arthritis (sJIA) and to determine the involvement of these miRNA in regulating the expression of cytokines.<bold>Methods: </bold>Microarray profiling was performed to identify differentially expressed miRNA in sJIA plasma. Levels of candidate miRNA and mRNA were assessed by real-time PCR, and cytokines were measured by ELISA. Dual-luciferase reporter assay was used to validate the direct interaction between miR-26a and interleukin 6 (IL-6).<bold>Results: </bold>Forty-eight miRNA were differentially expressed in the plasma of patients with sJIA compared with healthy controls (HC). Five miRNA were selected for further validation. The expression level of miR-26a was exclusively elevated in the plasma of patients with sJIA as compared with 4 rheumatic diseases and 2 subtypes of JIA (oligoarticular and polyarticular). The levels of IL-6, IL-1β, and tumor necrosis factor-α in the plasma of patients with sJIA were increased, and only IL-6 presented a positive correlation with miR-26a (r = 0.539, p < 0.0001). After stimulation with IL-6, miR-26a expression was upregulated in THP-1 cells, while the supernatant level of IL-6 was downregulated by transfection of miR-26a mimics. Consistently, direct target relationship between miR-26a and IL-6 was confirmed.<bold>Conclusion: </bold>This study demonstrates that miR-26a is expressed specifically and highly in sJIA plasma and suggests that miR-26a may regulate the levels of cytokines in sJIA. Our findings highlight miR-26a as a potential biomarker for the diagnosis as well as differential diagnosis of sJIA.
- Subjects
CYTOKINES; JUVENILE idiopathic arthritis; RNA; DIAGNOSIS
- Publication
Journal of Rheumatology, 2016, Vol 43, Issue 8, p1607
- ISSN
0315-162X
- Publication type
journal article
- DOI
10.3899/jrheum.150593