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- Title
Evidence of oxidative stress in brain and liver of young rats submitted to experimental galactosemia.
- Authors
Castro, Márcia; Ferreira, Bruna; Cararo, José; Chipindo, Adália; Magenis, Marina; Michels, Monique; Danielski, Lucinéia; Oliveira, Marcos; Ferreira, Gustavo; Streck, Emilio; Petronilho, Fabricia; Schuck, Patrícia
- Abstract
Galactosemia is a disorder of galactose metabolism, leading to the accumulation of this carbohydrate. Galactosemic patients present brain and liver damage. For evaluated oxidative stress, 30-day-old males Wistar rats were divided into two groups: galactose group, that received a single injection of this carbohydrate (5 μmol/g), and control group, that received saline 0.9 % in the same conditions. One, twelve or twenty-four hours after the administration, animals were euthanized and cerebral cortex, cerebellum, and liver were isolated. After one hour, it was found a significant increase in TBA-RS levels, nitrate and nitrite and protein carbonyl contents in cerebral cortex, as well as protein carbonyl content in the cerebellum and in hepatic level of TBA-RS, and a significant decrease in nitrate and nitrite contents in cerebellum. TBA-RS levels were also found increased in all studied tissues, as well as nitrate and nitrite contents in cerebral cortex and cerebellum, that also present increased protein carbonyl content and impairments in the activity of antioxidant enzymes of rats euthanized at twelve hours. Finally, animals euthanized after twenty-four hours present an increase of TBA-RS levels in studied tissues, as well as the protein carbonyl content in cerebellum and liver. These animals also present an increased nitrate and nitrite content and impairment of antioxidant enzymes activities. Taken together, our data suggest that acute galactose administration impairs redox homeostasis in brain and liver of rats.
- Subjects
GALACTOSEMIA; OXIDATIVE stress; PHYSIOLOGICAL effects of galactose; ANTIOXIDANTS; CARBONYL group; CEREBRAL cortex; BRAIN physiology; LIVER physiology; THERAPEUTICS
- Publication
Metabolic Brain Disease, 2016, Vol 31, Issue 6, p1381
- ISSN
0885-7490
- Publication type
Article
- DOI
10.1007/s11011-016-9865-3