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- Title
Renal contrast microangiography with synchrotron radiation: a novel method for visualizing structures within nephrons in vivo.
- Authors
Miya, Ken; Matsushita, Shonosuke; Hyodo, Kazuyuki; Tokunaga, Chiho; Sakamoto, Hiroaki; Mizutani, Taro; Hiramatsu, Yuji
- Abstract
Background No non-invasive method of observing renal microcirculation in vivo has been established as yet. Although angiography is considered to be ideally suited for the purpose, conventional X-rays cannot be used to image structures smaller than 100 µm. Purpose To develop a method for visualizing the renal arterioles, glomeruli, and proximal tubules of rats in vivo making use of synchrotron radiation. Material and Methods Male Wistar rats were anesthetized, and a catheter was inserted via laparotomy into the abdominal aorta with its tip placed above the renal arteries. The rats were paralyzed with a neuromuscular blocking agent and mechanically ventilated. An inorganic iodine contrast medium was injected via the catheter. The SR derived X-rays transmitted through the subjects were recorded with a CCD camera. Two-dimensional images with a pixel size of 9 µm were obtained. The exposure time was fixed at 50 ms, with a maximum acquisition rate of three images/s. Results Renal arterioles as small as 18 µm in diameter, glomeruli with an average diameter of 173 ± 21 µm, as well as proximal tubules, were clearly visualized. In addition, glomerular density at the peripheral renal cortex was measurable. Conclusion Rat renal microcirculation could be successfully observed in real-time, without exteriorization of the kidney in this study.
- Subjects
SYNCHROTRON radiation; NEPHRONS; ABDOMINAL surgery; RENAL artery; KIDNEY glomerulus; ABDOMINAL aorta; RADIOGRAPHY equipment; DIAGNOSTIC imaging equipment; ANGIOGRAPHY; ANIMAL experimentation; ARTERIES; DIAGNOSTIC imaging; KIDNEYS; MICROCIRCULATION; PARTICLE accelerators; RADIOGRAPHY; RATS; CONTRAST media; EQUIPMENT &; supplies
- Publication
Acta Radiologica, 2017, Vol 58, Issue 4, p505
- ISSN
0284-1851
- Publication type
journal article
- DOI
10.1177/0284185116658685