We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Characterization of Cellobiohydrolases from Schizophyllum commune KMJ820.
- Authors
Kondaveeti, Sanath; Patel, Sanjay K. S.; Woo, Janghun; Wee, Ji Hyang; Kim, Sang-Yong; Al-Raoush, Riyadh I.; Kim, In-Won; Kalia, Vipin Chandra; Lee, Jung-Kul
- Abstract
A novel cellobiohydrolase (CBH)-generating fungi have been isolated and categorized as Schizophyllum commune KMJ820 based on morphology and rDNA gene sequence. Cellulose powder was used as carbon source, the total enzyme activity was 11.51 U/ml is noted; which is among the highest amounts of CBH-generating microbes studied. CBH have been purified to homogenize, with pursual of serial chromatography using S. commune supernatants and two different CBHs were found; CBH 1 and 2. The filtered CBHs showed greater activity (Vmax = 51.4 and 20.8 U/mg) in contrast to CBHs from earlier studies. The MW (molecular weights) of S. commune CBH 1 and 2 were verified to be approximately 50 kDa and 150 kDa, respectively, by size exclusion chromatography. Even though CBHs have been evaluated from other sources, but S. commune CBH is prominent in comparison to other CBHs by its high enzyme activity.
- Subjects
GEL permeation chromatography; COMMUNAL living; MOLECULAR weights; CELLULOSE 1,4-beta-cellobiosidase
- Publication
Indian Journal of Microbiology, 2020, Vol 60, Issue 2, p160
- ISSN
0046-8991
- Publication type
Article
- DOI
10.1007/s12088-019-00843-9