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- Title
Matrix metalloproteinases and TIMP-1 production by peripheral blood granulocytes from COPD patients and asthmatics.
- Authors
Cataldo, D.; Munaut, C.; Noël, A.; Frankenne, F.; Bartsch, P.; Foidart, J.M.; Louis, R.
- Abstract
Both asthmatic and COPD patients were found to have increased amounts of granulocytes and matrix metalloproteinase-9 (MMP-9) in their sputum. The present study was conducted to investigate whether the elevated amounts of MMP-9 and TIMP-1 found in such patients' airways may be linked to an enhanced secretion by granulocytes. Blood granulocytes from asthmatics (n=10), COPD patients (n=11), and healthy controls (n=11) were isolated and cultured under basal conditions or after stimulation by phorbol 12-myristate 13- acetate (PMA) or N-formyl-L-methionyl-L-leucyl-L-phenylalanine (fMLP). MMP-9 activity was detected by zymography while MMP-8 and TIMP-1 levels were measured by ELISA. In zymography, pro- and activated forms of MMP-9 were present in each group (healthy subjects, asthmatics, and COPD patients). Spontaneous release was not different between the three groups. Stimulation by fMLP and PMA increased to a similar extent the release of MMP-9 by granulocytes in all the three groups. TIMP-1 levels were also increased after stimulation by PMA and fMLP only in healthy subjects and COPD patients. MMP-8 levels were barely detectable. We conclude that circulating granulocytes from COPD patients and asthmatics do not display an abnormal secretion of MMP-9, and that granulocytes from asthmatics have an impaired ability to release TIMP-1 upon stimulation.
- Subjects
METALLOPROTEINASES; GRANULOCYTES
- Publication
Allergy, 2001, Vol 56, Issue 2, p145
- ISSN
0105-4538
- Publication type
Article
- DOI
10.1034/j.1398-9995.2001.056002145.x