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- Title
Comparative Analysis of Enzymatic Transglycosylation Using E. coli Nucleoside Phosphorylases: A Synthetic Concept for the Preparation of Purine Modified 2′-Deoxyribonucleosides from Ribonucleosides.
- Authors
Drenichev, Mikhail S.; Oslovsky, Vladimir E.; Zenchenko, Anastasia A.; Danilova, Claudia V.; Varga, Mikhail A.; Esipov, Roman S.; Lykoshin, Dmitry D.; Alexeev, Cyril S.
- Abstract
A comparative analysis of the transglycosylation conditions catalyzed by E. coli nucleoside phosphorylases, leading to the formation of 2′-deoxynucleosides, was performed. We demonstrated that maximal yields of 2′-deoxynucleosides, especially modified, can be achieved under small excess of glycosyl-donor (7-methyl-2′-deoxyguanosine, thymidine) and a 4-fold lack of phosphate. A phosphate concentration less than equimolar one allows using only a slight excess of the carbohydrate residue donor nucleoside to increase the reaction's output. A three-step methodology was elaborated for the preparative synthesis of purine-modified 2′-deoxyribonucleosides, starting from the corresponding ribonucleosides.
- Subjects
RIBONUCLEOSIDES; ENZYMATIC analysis; COMPARATIVE studies; CARBOHYDRATES; THYMIDINE; PHOSPHORYLASES
- Publication
International Journal of Molecular Sciences, 2022, Vol 23, Issue 5, p2795
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms23052795