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- Title
In vitro culture of Gigaspora decipiens and Glomus clarum in transformed roots of carrot: the influence of temperature and pH.
- Authors
Araújo Costa, Francilina; Meira Haddad, Lydice Sant’Anna; Megumi Kasuya, Maria Catarina; Campos Oton, Wagner; Dutra Costa, Maurício; Chaer Borges, Arnaldo
- Abstract
Monoxenic cultures of arbuscular mycorrhizal fungi (AMF) in transformed roots have been used to better understand the symbiosis with these fungi, but few species have been successfully established in vitro. The object was to establish monoxenic cultures of Gigaspora decipiens and Glomus clarum and to verify the effects of temperature and pH on spore formation. Cultures were established from superficially disinfested spores that were germinated on an agar and water. After germination, the spores were transferred to Petri dishes containing transformed carrot roots. After 4-6 days formed newly formed spores and structures typical. The effects of temperature (22, 25, 28 or 32°C) and pH (4.0, 4.5, 5.5 or 6.5) on the production of spores were assessed over three months, resulting in sigmoidal growth curves. The spore increased from 22°C, reaching higher values 28oC and 32oC there was a reduction in the production. The highest spore production of G. decipiens occurred in a pH of 6.5, whereas for G. clarum was pH of 4.0. The cultivation of other species is still necessary to elucidate particular aspects of the symbiosis that so far are unclear, including the effects of environmental factors on the production of spores of different AMF species.
- Subjects
VESICULAR-arbuscular mycorrhizas; PLANT fertilization; CARROTS; PLANT roots; EFFECT of temperature on plants
- Publication
Acta Scientiarum: Agronomy, 2013, Vol 35, Issue 3, p315
- ISSN
1679-9275
- Publication type
Article
- DOI
10.4025/actasciagron.v35i3.16581