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- Title
Different alpha crystallin expression in human age-related and congenital cataract lens epithelium.
- Authors
Jing Yang; Sheng Zhou; Minfei Guo; Yuting Li; Jianjun Gu; Yang, Jing; Zhou, Sheng; Guo, Minfei; Li, Yuting; Gu, Jianjun
- Abstract
<bold>Background: </bold>The purpose of this study was to investigate the different expressions of αA-crystallin and αB-crystallin in human lens epithelium of age-related and congenital cataracts.<bold>Methods: </bold>The central part of the human anterior lens capsule approximately 5 mm in diameter together with the adhering epithelial cells, were harvested and processed within 6 hours after cataract surgery from age-related and congenital cataract patients or from normal eyes of fresh cadavers. The mRNA and soluble protein levels of αA-crystallin and αB-crystallin in the human lens epithelium were detected by real-time PCR and western blots, respectively.<bold>Results: </bold>The mRNA and soluble protein expressions of αA-crystallin and αB-crystallin in the lens epithelium were both reduced in age-related and congenital cataract groups when compared with the normal control group. However, the degree of α-crystallin loss in the lens epithelium was highly correlated with different cataract types. The α-crystallin expression of the lens epithelium was greatly reduced in the congenital cataract group but only moderately decreased in the age-related cataract group. The reduction of αA-crystallin soluble protein levels in the congenital cataract group was approximately 2.4 fold decrease compared with that of the age-related cataract group, while an mRNA fold change of 1.67 decrease was observed for the age-related cataract group. Similarly, the reduction of soluble protein levels of αB-crystallin in the congenital cataract group was approximately a 1.57 fold change compared with that of the age-related cataract group. A 1.75 fold change for mRNA levels compared with that of the age-related cataract group was observed.<bold>Conclusions: </bold>The results suggest that the differential loss of α-crystallin in the human lens epithelium could be associated with the different mechanisms of cataractogenesis in age-related versus congenital cataracts, subsequently resulting in different clinical presentations.
- Subjects
ALPHA-B-crystallin; AGE factors in cataracts; EPITHELIUM; MESSENGER RNA; EPITHELIAL cells; PROTEIN metabolism; RNA metabolism; ANALYSIS of variance; CATARACT; CRYSTALLINE lens; POLYMERASE chain reaction; WESTERN immunoblotting
- Publication
BMC Ophthalmology, 2016, Vol 16, p1
- ISSN
1471-2415
- Publication type
journal article
- DOI
10.1186/s12886-016-0241-1