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- Title
Simultaneous determination of seven sesquiterpene lactone glucosides in Ixeris dentata by high-performance liquid chromatography coupled with tandem mass spectrometry and their antiviral activities.
- Authors
Park, SeonJu; Kim, Nanyoung; Park, Jun Hyung; Lee, Sang-Won; Song, Jae-Hyoung; Ko, Hyun-Jeong; Chae, Han-Jung; Kim, Hyung-Ryong; Kim, Seung Hyun
- Abstract
Ixeris dentata (Thunb. ex Thunb.) Nakai (Asteraceae), a well-known edible vegetable in Asia, contains various bioactive secondary metabolites, including sesquiterpene lactones. In this study, a high-performance liquid chromatography–tandem mass spectrometry (HPLC–MS/MS) method has been developed and validated for simultaneous determination of seven sesquiterpene lactone glucosides isolated from the roots of I. dentata. In addition, these compounds were evaluated in terms of their antiviral activities against coxsackievirus B3 (CVB3) and human enterovirus 71 (EV71). The developed method was validated in terms of linearity (R2 > 0.9996), precision (RSD < 2.24%), accuracy (96.30–102.77%), and stability (RSD < 1.94%) and successfully applied to the quantitation of the I. dentata root samples collected from six different regions of Korea. The content of sesquiterpene lactone glucosides varied significantly based on the region. For the antiviral activities, guaianolides with an ester group at C-8 (compounds 6 and 7) showed the most potent activities against CBV3, while germacranolide (compound 5) showed the most consistent antiviral activity against both CVB3 and EV71. The method was validated to be simple and reliable to simultaneously determine seven putative bioactive sesquiterpene lactone glucosides, the substantial chemotaxonomic markers, in I. dentata root samples.
- Subjects
SOUTH Korea; ASIA; TANDEM mass spectrometry; LIQUID chromatography-mass spectrometry; HIGH performance liquid chromatography; GLUCOSIDES; METABOLITES; SESQUITERPENE lactones
- Publication
Acta Chromatographica, 2019, Vol 31, Issue 4, p280
- ISSN
1233-2356
- Publication type
Article
- DOI
10.1556/1326.2018.00470