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- Title
Isolation and Purification of Two Tyrosinase Inhibitors from Camellia Pollen by High-Speed Counter-Current Chromatography.
- Authors
Yang, Y.-F.; Lai, X.-Y.; Huang, G.-L.; Chen, Y.-H.; Du, X.-P.; Jiang, Z.-D.; Chen, F.; Ni, H.
- Abstract
Bee pollen is a health food with a wide range of nutritional and therapeutic properties. However, the bioactive compounds of bee pollen have not been extensively revealed due to low efficacy in separation. High-speed counter-current chromatography (HSCCC) and solvent extraction were applied to separate tyrosinase inhibitors from camellia pollen in this study. The camellia pollen extracts prepared with petroleum ether, ethyl acetate, and n-BuOH have tyrosinase inhibitory activity. Acidic hydrolysis could promote the tyrosinase inhibitory activity of crude sample. Three fractions with tyrosinase inhibitory activity were separated from the hydrolysate by a one-step HSCCC procedure. Among the fractions, two chemicals were sufficiently purified and identified to be levulinic acid (LA) and 5-hydroxymethylfurfural (5-HMF). The recovery was 0.80 g kg-1 pollen for LA and 1.75 g kg-1 pollen for 5-HMF; and their purity was all over 98%. The study demonstrates that HSCCC method is powerful for preparative separation of tyrosinase inhibitors from camellia pollen.
- Subjects
PHENOL oxidase; ENZYME inhibitors; CHEMICAL purification; CAMELLIAS; COUNTERCURRENT chromatography; BIOACTIVE compounds; NATURAL foods
- Publication
Acta Chromatographica, 2017, Vol 29, Issue 4, p477
- ISSN
1233-2356
- Publication type
Article
- DOI
10.1556/1326.2017.29407