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- Title
Actin filament associated protein mediates c-Src related SRE/AP-1 transcriptional activation
- Authors
Han, Bing; Xiao, Helan; Xu, Jing; Lodyga, Monika; Bai, Xiao-Hui; Jin, Tianru; Liu, Mingyao
- Abstract
Abstract: AFAP is an adaptor protein involved in cytoskeletal organization and intracellular signaling. AFAP binds and activates c-Src; however, the downstream signals of this interaction remain unknown. Here we show that co-expression of AFAP and c-Src induce transcriptional activation of SRE and AP-1 in a c-Src activity dependent fashion. Structural-functional studies suggest that the proline-rich motif in the N-terminus of AFAP is critical for c-Src activation, and subsequent SRE/AP-1 transactivation and the actin-binding domain in the AFAP C-terminus is negatively involved in the regulation of AFAP/c-Src mediated SRE/AP-1 transactivation. Selective deletion of this domain enhances transactivation of SRE. We conclude that in addition to its role in the regulation of cytoskeletal structures, AFAP may also be involved in the c-Src related transcriptional activities. Structured summary: Src physically interacts with AFAP by anti bait coimmunoprecipitation (View interaction)
- Subjects
ACTIN; CELLULAR signal transduction; CYTOSKELETON; LEUCINE zippers; GENE expression; PROTEIN binding
- Publication
FEBS Letters, 2011, Vol 585, Issue 3, p471
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2011.01.014