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- Title
The polyketide cyclase RemF from Streptomyces resistomycificus contains an unusual octahedral zinc binding site
- Authors
Silvennoinen, Laura; Sandalova, Tatyana; Schneider, Gunter
- Abstract
Abstract: RemF is a polyketide cyclase involved in the biosynthesis of the aromatic pentacyclic metabolite resistomycin in Streptomyces resistomycificus. The enzyme is a member of a structurally hitherto uncharacterized class of polyketide cyclases. The crystal structure of RemF was determined by SAD and refined to 1.2Å resolution. The enzyme subunit shows a β-sandwich structure with a topology characteristic for the cupin fold. RemF contains a metal binding site located at the bottom of the predominantly hydrophobic active site cavity. A zinc ion is coordinated to four histidine side chains, and two water molecules in octahedral ligand sphere geometry, highly unusual for zinc binding sites in proteins.
- Subjects
POLYKETIDES; STREPTOMYCES; ZINC; BINDING sites; METABOLITES; BIOSYNTHESIS; ENZYMES; MEMBRANE proteins
- Publication
FEBS Letters, 2009, Vol 583, Issue 17, p2917
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2009.07.061