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- Title
Alternative splicing generates a novel non-secretable resistin isoform in Wistar rats<sup>1</sup><FN ID="FN1"><NO>1</NO>GenBank accession number of S-resistin AJ555618.</FN>
- Authors
Del Arco, A.; Peralta, S.; Carrascosa, J.M.; Ros, M.; Andrés, A.; Arribas, C.
- Abstract
Resistin is a secreted adipose tissue hormone that belongs to the resistin-like molecule family. We report here a new alternatively spliced isoform of the rat resistin gene, named S-resistin (short resistin), detected in adipose tissue by reverse transcription-polymerase chain reaction (RT-PCR). A comparison of this cDNA variant and genomic sequences indicates the lack of the second exon containing the secretory consensus signal. Both cDNAs, resistin and S-resistin, were carboxy-tagged with FLAG epitope and transiently expressed in cultured cell lines. While the resistin-FLAG construct gives the expected pattern for a secretion protein, the S-resistin-FLAG construct yielded a predominant nuclear staining. These results indicate that this splicing event regulates the fate and probably the function of the mature protein.
- Subjects
ADIPOSE tissues; HORMONES; GENES; POLYMERASE chain reaction
- Publication
FEBS Letters, 2003, Vol 555, Issue 2, p243
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/S0014-5793(03)01241-9