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- Title
Leptin receptor isoform expression in rat osteoblasts and their functional analysis
- Authors
Lee, Yun-Jung; Park, Jung-Hyun; Ju, Sung-Kyu; You, Kwan-Hee; Ko, Jea Seung; Kim, Hyun-Man
- Abstract
The genetic defect in producing the adipose hormone leptin results among others in a drastic increase of bone mass. The current understanding is that under normal circumstances, osteoblast activity is indirectly suppressed by a hypothalamic relay induced by leptin-signalling in the brain. To investigate whether leptin might also regulate osteoblast activity in a direct manner, expression of leptin receptors in rat osteoblasts was determined and their functionality was analyzed upon recombinant leptin treatment. Reverse transcription-PCR confirmed the expression of four among the six currently described receptor isoforms, which were also able to transduce cell signalling as shown by STAT3 phosphorylation after activation.
- Subjects
LEPTIN; FLOW cytometry; PHOSPHORYLATION
- Publication
FEBS Letters, 2002, Vol 528, Issue 1-3, p43
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/S0014-5793(02)02889-2