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- Title
Expression of the MSI-99m Gene in Transgenic Potato Plants Confers Resistance to Phytophthora infestans and Ralstonia solanacearum.
- Authors
Hong, Yong-bo; Liu, Shui-ping; Zhu, Yan-ping; Xie, Chao; Jue, Deng-wei; Chen, Min; Kaleri, Hubdar; Yang, Qing
- Abstract
Magainins are a class of antimicrobial peptides isolated from skin secretions of the African clawed frog Xenopus laevis. MSI-99 is a synthesized magainin II analogue with high inhibitory effects to a wide spectrum of microbial organisms, including bacteria, fungi and viruses. To verify the resistance conferred by the MSI-99m gene (a MSI-99 synthetic gene with codon usage adapted for expression in potato) to potato pathogens and to generate multi-resistant potato materials, we transferred the MSI-99m gene into potato plants using Agrobacterium-mediated transformation. PCR and Southern blot analyses of eight kanamycin-resistant plants showed that MSI-99m gene was present and expressed in five independent transgenic lines. These five transgenic plants exhibited enhanced resistance to Phytophthora infestans and Ralstonia solanacearum. The results demonstrate that the MSI-99m gene can be used to potentially improve potato disease resistance genetically.
- Subjects
GENE expression in plants; TRANSGENIC plants; POTATO disease &; pest resistance; PHYTOPHTHORA infestans; MAGAININS; GENETIC code; PEPTIDE antibiotics; XENOPUS laevis
- Publication
Plant Molecular Biology Reporter, 2013, Vol 31, Issue 2, p418
- ISSN
0735-9640
- Publication type
Article
- DOI
10.1007/s11105-012-0503-x