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- Title
Expression of the Human Tissue-Plasminogen Activator in Hairy Roots of Oriental Melon ( Cucumis melo).
- Authors
Kang, Sol; Ajjappala, Hemavathi; Seo, Hyun-Hui; Sim, Joon-Soo; Yoon, Sang-Hong; Koo, Bon-Sung; Kim, Yong-Hwan; Lee, Sukchan; Hahn, Bum-Soo
- Abstract
The human tissue plasminogen activator (t-PA) is a thrombolytic protein that plays a key role in fibrinolysis by converting the plasminogen into plasmin which degrades fibrin clots in blood vessels. The t-PA gene encoding the human tissue plasminogen activator was cloned and expressed under transcriptional control of single and multiple rooting loci promoter ( rolD) promoters in hairy roots derived from Oriental melon. t-PA gene integration in the genome of hairy roots was confirmed by PCR. Increased transcript and protein expression levels was observed in the transgenic hairy roots expressing t-PA under the control of single and dual rolD promoters, respectively. Enzyme-linked immunosorbent assay revealed the highest levels of the recombinant t-PA accumulation in transgenic hairy roots carrying the t-PA transgene under the control of single and dual rolD (0.17-0.15 μg/mg) promoters as compared to triple and quadruple rolD (0.07-0.03 μg/mg) promoters. Fibrinolytic activity indicated the proper function of the expressed recombinant t-PA in fibrin lysis. These results demonstrated that hairy roots could serve as alternatives to transgenic plants for expression of mammalian genes.
- Subjects
GENE expression in plants; TISSUE plasminogen activator; PLANT roots; MELONS; PROMOTERS (Genetics); FIBRINOLYSIS; TRANSCRIPTION factors; ENZYME-linked immunosorbent assay
- Publication
Plant Molecular Biology Reporter, 2011, Vol 29, Issue 4, p919
- ISSN
0735-9640
- Publication type
Article
- DOI
10.1007/s11105-011-0303-8