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- Title
Screening of AMP-activated protein kinase α2 subunit interacting proteins by bacterial two-hybrid system.
- Authors
Qing-Ying Fu; Yu-Qi Gao
- Abstract
Abstract AMP-activated protein kinase (AMPK) is a ubiquitous eukaryotic protein kinase regulating cellular metabolism and energy demand. In brain AMPK plays a role as a multidimensional energy sensor and AMPK α2 subunit is expressed at higher levels than AMPK α1 subunit. In order to identify potential targets of AMPK in brain, we perform bacterial two-hybrid screening of a rat fetal brain cDNA library using AMPK α2 subunit as bait. Here, we present seven potential AMPK α2 subunit interacting proteins, including 6-phosphofructo-1-kinase (PFK-1), polyubiquitin, cytochrome c oxidase subunit I (COX I), heat shock protein 8 (HSP8), HLA-B-associated transcript 3 (BAT3) isoform 1, protein tyrosine phosphatase receptor type D (PTPRD) and islet-brain 1 (IB1). They are involved in glycolysis, protein degradation, mitochondrial electron transport and apoptosis pathways participating in energy regulation directly or indirectly. These data may provide new insight into further studying the pathways of AMPK energy regulation in brain and possible mechanisms of AMPK-mediated neuroprotective effect.
- Subjects
PROTEIN kinases; PHOSPHOTRANSFERASES; CYCLIN-dependent kinases; GLYCOGEN synthase kinase-3
- Publication
Molecular Biology Reports, 2009, Vol 36, Issue 2, p337
- ISSN
0301-4851
- Publication type
Article
- DOI
10.1007/s11033-007-9184-1