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- Title
Comparison of polimerase chain reaction and serological methods in the diagnosis of hepatitis C virus infection.
- Authors
Kaya, Selçuk; Cicioğlu-Arıdoğan, Buket; Çetin, Emel Sesli; Adiloğlu, Ali K.; Demirci, Mustafa
- Abstract
Diagnosis of HCV infection depends on detecting anti-HCV antibody (HCV-Ab) through serologic methods or on detecting of HCV RNA with molecular methods. The aim of this study was to evaluate the use of serological methods and polymerase chain reaction (PCR) for the diagnosis of HCV infection. A total of 87 patients were studied. HCV RNA was detected by real time PCR with GeneAmp 5700 Sequence Detection System. Thirty (34.5%) of 87 patients were HCV RNA positive and 60 (69%) were HCV-Ab positive. Fourteen of the HCV-Ab positive patients had low antibody titers (below 2.5 U/ml). None of these was found as HCV RNA positive. All of HCV-Ab negative patients were HCV RNA negative and all of the HCV RNA positive cases were HCV-Ab positive also. Since HCV RNA was negative in all of HCV Ab negative patients, it can be suggested that HCV-Ab detection by a reliable ELISA technique in the routine procedure is sufficient to determine HCV infection . On the other hand, low titer positive HCV-Ab cases must be restudied by ELISA, and PCR for HCV RNA confirmation. By using ELISA and PCR methods at the same time would help to confirm the positive results and early diagnosis.
- Subjects
LIVER disease diagnosis; ALCOHOLIC liver diseases; POLYMERASE chain reaction; HEPATITIS C; RNA; ENZYME-linked immunosorbent assay
- Publication
Medical Journal of Suleyman Demirel University, 2007, Vol 14, Issue 1, p10
- ISSN
1300-7416
- Publication type
Article