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- Title
Isolation of a maize ZmCI- 1B promoter and characterization of its activity in transgenic maize and tobacco.
- Authors
Li, Ye; Liu, Xiaoqing; Li, Jie; Li, Suzhen; Chen, Guanyu; Zhou, Xiaojin; Yang, Wenzhu; Chen, Rumei
- Abstract
Key message: The 2-kb ZmCI - 1B promoter is active in the root and embryo and induced by wounding in maize and the 220-bp 5′-deleted segment maybe the minimal promoter. Abstract: The subtilisin-chymotrypsin inhibitor gene, CI-1B of Zea mays ( ZmCI- 1B), has been suggested to induce the maize defense system to resist insect attack. Real-time RT-PCR showed that ZmCI- 1B gene exhibited especially high expression in roots and embryos. The 2-kb full-length promoter of ZmCI- 1B gene was isolated from the maize genome and used to drive expression of a beta-glucuronidase ( GUS) reporter gene for transient expression and stable expression analysis in maize. The results of GUS histochemical staining in transgenic maize plants revealed that the ZmCI- 1B promoter induced GUS expression preferentially in roots and embryos and in response to wounding. A series of 5′-deleted segments of the ZmCI- 1B promoter were cloned individually to drive GUS expression for further analysis. Deletion analysis combined with the histochemical staining of transgenic tobacco plants revealed 220-bp segment could drive GUS in a tissue-specific and wounding-induced expression in tobacco; thus, it maybe the minimally active promoter of ZmCI- 1B gene. Furthermore, it revealed that the ZmCI- 1B promoter contained tissue-specific and wounding-induced elements.
- Subjects
CORN genetics; PLANT embryology; GENE expression in plants; GLUCURONIDASE genetics; PLANT roots
- Publication
Plant Cell Reports, 2015, Vol 34, Issue 8, p1443
- ISSN
0721-7714
- Publication type
Article
- DOI
10.1007/s00299-015-1799-4