We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
The 2A2 protein of Duck hepatitis A virus type 1 induces apoptosis in primary cell culture.
- Authors
Cao, Jingyu; Ou, Xumin; Zhu, Dekang; Ma, Guangpeng; Cheng, Anchun; Wang, Mingshu; Chen, Shun; Jia, Renyong; Liu, Mafeng; Sun, Kunfeng; Yang, Qiao; Wu, Ying; Chen, Xiaoyue
- Abstract
Duck hepatitis A virus type 1, (DHAV-1) 2A2, is one of the most highly conserved viral proteins within the DHAV serotypes. However, its effect on host cells is unclear. We predicted that DHAV-1 2A2 was a GTPase-like protein based on the results of multiple sequence alignment and homologous modeling analysis. Upon transfection of a recombinant plasmid expressing DHAV-1 2A2, cells displayed fragmented nuclei, chromatin condensation, oligonucleosome-sized DNA ladder, and positive terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling staining; hence, cell death has the characteristics of apoptosis. By staining cells with fluorescein Annexin V-FITC and PI, it is possible to distinguish and quantitatively analyze nonapoptotic cells, early apoptotic cells, late apoptotic/necrotic cells, and dead cells through flow cytometry and fluorescence microscopy. The percentage of apoptotic cells gradually increased and reached a maximum after 48 h of transfection. In conclusion, apoptosis induced by this GTPase-like protein may contribute to DHAV-1 pathogenesis.
- Subjects
DUCK hepatitis B virus; HEPATITIS viruses; GUANOSINE triphosphatase; VIRAL proteins; APOPTOSIS
- Publication
Virus Genes, 2016, Vol 52, Issue 6, p780
- ISSN
0920-8569
- Publication type
Article
- DOI
10.1007/s11262-016-1364-4