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- Title
A reliable method to avoid contamination during cartilage graft preparation in septorhinoplasty.
- Authors
Arslan, Fatih; Ocal, Duygu; Yildiz, Cemile Acikgoz; Dolapci, Iştar
- Abstract
Purpose: The aim of the study is to determine the risk of contamination in the cartilage graft materials prepared on the swester table and those prepared in a sterile package, and to reveal a more reliable method by performing the microbiological examination of these materials. Methods: Cartilages removed from the nasal septum were divided into four pieces. The first part (Sample A) was directly placed into the medium. Sample B was prepared by being crushed in a sterile package. Sample C was prepared on the auxiliary swester table, and Sample D was prepared on the main swester table actively used by surgery team. All samples were transferred in a 1 ml brain heart(BH) liquid medium. From each BH medium, 100 µl culture was performed on blood agar, eosin–methylene blue–lactose–sucrose agar and chocolate agar. Results: Bacterial growth was detected in 2 of the samples A, in 4 of the samples B, in 24 of the samples C, and in 36 of the samples D. The number of patients with bacterial growth in the samples C and/or D despite no growth in the sample B was 35. When the samples A/B and C/D were compared in terms of bacterial growth, a significant difference was found in all matchings (p < 0.001 for all comparisons). Conclusion: These findings showed that preparation of the cartilage grafts on the swester table was extremely risky for microbiological contamination. Arslan and his colleagues suggest that preparing a graft material in a sterile package is extremely simple, cheap, and it also reduces contamination risk significantly.
- Subjects
MICROBIAL contamination; CARTILAGE; BACTERIAL growth; NASAL septum; AGAR
- Publication
European Archives of Oto-Rhino-Laryngology, 2019, Vol 276, Issue 5, p1385
- ISSN
0937-4477
- Publication type
Article
- DOI
10.1007/s00405-019-05342-7