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- Title
A simple graphene oxide‐based DNA purification strategy for plant pathogen detection.
- Authors
Zhang, Jing; Wang, Xiaochang; Ao, Ningjing; Zou, Huayan; Li, Jingwei; Shao, Huijuan; Kageyama, Koji; Feng, Wenzhuo
- Abstract
BACKGROUND: The on‐site molecular detection of plant pathogens is particularly important for the development of sustainable agriculture. Extracting DNA from plant tissues, microbes or coexisting environments is complex, labor‐intensive and time‐consuming. To facilitate this process, we propose a DNA purification strategy based on graphene oxide (GO). RESULTS: The excellent adsorption ability of GO was verified by visualizing changes in its microscopic surface and macroscopic mixture. To further optimize the DNA purification, we determined the optimal GO concentration and treatment time at 95 °C (2 mg mL−1 and 2 min, respectively). We confirmed that our strategy is effective on plant tissues and various microorganisms, and that the obtained DNA can be directly used for polymerase chain reaction amplification. Combining the proposed GO‐based DNA purification method with the loop‐mediated isothermal amplification method is superior, in terms of the required steps, time, cost and detection effect, to the cetyltrimethylammonium bromide method and a commercial kit for detecting plant pathogens. CONCLUSION: We present a feasible, rapid, simple and low‐cost DNA purification method with high practical value for scientific applications in plant pathogen detection. This strategy can also provide important technical support for future research on plant–microbial microenvironments. © 2024 Society of Chemical Industry.
- Subjects
SOCIETY of Chemical Industry (Great Britain); PHYTOPATHOGENIC microorganisms; DNA; GRAPHENE; SUSTAINABLE agriculture; SUSTAINABLE development; PLANT DNA
- Publication
Pest Management Science, 2024, Vol 80, Issue 7, p3516
- ISSN
1526-498X
- Publication type
Article
- DOI
10.1002/ps.8056