We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Effect of α1-Acid Glycoprotein Expressed in Cancer Cells on Malignant Characteristics.
- Authors
Soo Young Lee; Joo Weon Lim; You-Mie Kim
- Abstract
The α1-acid glycoprotein (AAG) is a prototypical serum acute phase reactant in most mammalian species; it is synthesized mainly in liver parenchymal cells. Recently, we found that mRNAs of AAG were expressed in non-hepatic cancer cells., and the expression levels were regulated by the cytokines- IL-1, IL-6, and TNF-α. The functional role of AAG in non-hepatic cancer cells has not yet been established. In order to understand the functional role of the AAG expressed in HT- 29 cells, the cancer cells were transfected with cloned cDNA for AAG, or exposed to antisense oligodeoxynueleotide (ODN) for AAG. The colony-forming capacity, invasion, and adhesion to laminin of these transformed cancer cells were measured. Overexpression of AAG by transfection, and inhibition of the AAG expression by antisense ODNs were identified by Western blot as well as nested reverse transcriptase-polymerase chain reaction (nested RT-PCR), respectively. Results showed that the overexpression of AAG by transfection reduced colony-forming capacities, invasion, and adhesion to laminin of the cancer cells; on the other hand, the antisense ODN for AAG elevated colony-forming capacities, invasion, and adhesion to laminin of the cancer cells. These results suggest that AAG. expressed in cancer cells inhibited proliferation, invasion, and metastasis of the cancer cells.
- Subjects
GLYCOPROTEINS; LIVER cells; CANCER cells; MESSENGER RNA; POLYMERASE chain reaction
- Publication
Molecules & Cells (Springer Nature), 2001, Vol 11, Issue 3, p341
- ISSN
1016-8478
- Publication type
Article
- DOI
10.1016/s1016-8478(23)17045-2