We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
White-Light Supercontinuum Laser-Based Multiple Wavelength Excitation for TCSPC-FLIM of Cutaneous Nanocarrier Uptake.
- Authors
Volz, Pierre; Brodwolf, Robert; Zoschke, Christian; Haag, Rainer; Schäfer-Korting, Monika; Alexiev, Ulrike
- Abstract
We report here on a custom-built time-correlated single photon-counting (TCSPC)-based fluorescence lifetime imaging microscopy (FLIM) setup with a continuously tunable white-light supercontinuum laser combined with acousto-optical tunable filters (AOTF) as an excitation source for simultaneous excitation of multiple spectrally separated fluorophores. We characterized the wavelength dependence of the white-light supercontinuum laser pulse properties and demonstrated the performance of the FLIM setup, aiming to show the experimental setup in depth together with a biomedical application. We herein summarize the physical-technical parameters as well as our approach to map the skin uptake of nanocarriers using FLIM with a resolution compared to spectroscopy. As an example, we focus on the penetration study of indocarbocyanine-labeled dendritic core-multishell nanocarriers (CMS-ICC) into reconstructed human epidermis. Unique fluorescence lifetime signatures of indocarbocyanine-labeled nanocarriers indicate nanocarrier-tissue interactions within reconstructed human epidermis, bringing FLIM close to spectroscopic analysis.
- Subjects
NANOCARRIERS; PHOTON counting; SUPERCONTINUUM generation
- Publication
Zeitschrift für Physikalische Chemie, 2018, Vol 232, Issue 5-6, p671
- ISSN
0942-9352
- Publication type
Article
- DOI
10.1515/zpch-2017-1050