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- Title
Vicia faba SV channel VfTPC1 is a hyperexcitable variant of plant vacuole Two Pore Channels.
- Authors
Jinping Lu; Dreyer, Ingo; Dickinson, Miles Sasha; Panzer, Sabine; Jaślan, Dawid; Navarro-Retamal, Carlos; Geiger, Dietmar; Terpitz, Ulrich; Becker, Dirk; Stroud, Robert M.; Marten, Irene; Hedrich, Rainer
- Abstract
To fire action-potential-like electrical signals, the vacuole membrane requires the two-pore channel TPC1, formerly called SV channel. The TPC1/SV channel functions as a depolarization-stimulated, non-selective cation channel that is inhibited by luminal Ca2+. In our search for species-dependent functional TPC1 channel variants with different luminal Ca2+ sensitivity, we found in total three acidic residues present in Ca2+ sensor sites 2 and 3 of the Ca2+-sensitive AtTPC1 channel from Arabidopsis thaliana that were neutral in its Vicia faba ortholog and also in those of many other Fabaceae. When expressed in the Arabidopsis AtTPC1-loss-of-function background, wild-type VfTPC1 was hypersensitive to vacuole depolarization and only weakly sensitive to blocking luminal Ca2+. When AtTPC1 was mutated for these VfTPC1-homologous polymorphic residues, two neutral substitutions in Ca2+ sensor site 3 alone were already sufficient for the Arabidopsis At-VfTPC1 channel mutant to gain VfTPC1-like voltage and luminal Ca2+ sensitivity that together rendered vacuoles hyperexcitable. Thus, natural TPC1 channel variants exist in plant families which may fine-tune vacuole excitability and adapt it to environmental settings of the particular ecological niche.
- Subjects
PLANT vacuoles; FAVA bean; ARABIDOPSIS thaliana; ECOLOGICAL niche; LEGUMES; ARABIDOPSIS
- Publication
eLife, 2023, p1
- ISSN
2050-084X
- Publication type
Article
- DOI
10.7554/eLife.86384