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- Title
Investigation of Antimicrobial and Antioxidant Activities of Cirsium arvensis (L.) Scop From District Bhimber of Azad Jammu and Kashmir.
- Authors
Maqbool, Mehwish; Ajaib, Muhammad; Ishtiaq, Muhammad; Mushtaq, Waheeda; Azam, Shehzad; Haroon, Muhammad; Azam, Azhar; Shahzaman, Muhammad
- Abstract
Plants are part and parcel of human life. The current research was designed to explore ethnomedicinal importance of "Cirsium arvensis (L.) Scop." (family Asteraceae) from Samahni area of District Bhimber Azad Kashmir, Pakistan. The maceration protocol was applied using four solvents of different polarity and highest yield was found in water extract for leaf (9.85 mg) and for stem (8.50 mg) from 250 g dry wt of sample. Leaf extract produced significant quantity of biochemicals: saponin, terpnoids, alkaloids and tannins. The antimicrobial activity was explored using four bacterial strains and four fungal species. Petroleum extract (P.E.) macerate depicted the highest antibacterial and antimycotic activity. P.E. extract of leaf showed significant zone of inhibition (Z.I.) nearly 76±0.4 mm which was the most promising. For antimycotic, highest ZI was calculated for cholorformic extract of leaf (79±0.2 mm) against Aspergillus niger, followed by 77±0.7 mm for Fusarium oxysporum. Minimum inhibitory concentration (M.I.C.) was also determined for different bacterial and fungal strains. It was found 0.12±0.05 M.I.C. for E. coli and followed by 0.26±0.3 mm for B. subtilis. For antimycotic analysis, M.I.C. was determined significant for Aspergillus niger (0.05±0.3). In the study, activity index (A.I.) for bacteria was highest (4.22) for P.E. of leaf extract against E. coli and for fungi it was highest (3.59) for leaf chloroform extract against A. niger. The plant crude extract was also tested for total antioxidant activity (TAA) and it depicted 4.70±10.8 value for aqueous macerate. Other fractions also do have good range of TAA. In total phenolic content (TPC) analysis, highest TPC was determined for methanol extract of stem of for concentration of 125 mL with value of 78.93±0.3. These findings proved that plant is good source of herbal medication and it might be good source of novel drug discovery and development against bacteria and fungi.
- Subjects
ANTI-infective agents; CIRSIUM; ANTIFUNGAL agents; ASPERGILLUS niger; ESCHERICHIA coli
- Publication
Journal of the Chemical Society of Pakistan, 2017, Vol 39, Issue 1, p142
- ISSN
0253-5106
- Publication type
Article