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- Title
Evaluating the effectiveness of RNA in-situ hybridization for detecting lung adenocarcinoma with anaplastic lymphoma kinase rearrangement.
- Authors
Nakajima, Naoki; Yoshizawa, Akihiko; Kondo, Kyoko; Rokutan‐Kurata, Mariyo; Hirata, Masahiro; Furuhata, Ayako; Sumiyoshi, Shinji; Sonobe, Makoto; Menju, Toshi; Momose, Masanobu; Fujimoto, Masakazu; Date, Hiroshi; Haga, Hironori
- Abstract
Aims An easy and rapid assay for detecting mRNA in formalin-fixed paraffin-embedded samples [ RNA in-situ hybridization ( ISH)] has been reported recently. The aim of this study was to investigate the diagnostic accuracy of RNA ISH in detecting lung adenocarcinoma ( LA) with anaplastic lymphoma kinase ( ALK) gene rearrangement. Methods and results We tested ALK RNA ISH on 11 resected LAs for which ALK fusion was confirmed by immunohistochemistry ( IHC) and/or fluorescence in-situ hybridization ( FISH). ALK mRNA expression was detected by RNA ISH in all 11 ALK-positive LAs, with a mean positive cell proportion of 68.4% (median, 75.3%; range, 3-98.8%), by counting 100 tumour cells at 10 different loci; RNA ISH did not detect ALK mRNA expression in the normal surrounding lung cells. Next, we explored the concordance between ALK RNA ISH and IHC/ FISH tests by using tissue microarrays ( TMAs) containing 294 LAs. In the TMA slides, we found five ALK-positive cases with IHC and/or FISH. The mean proportion of ALK RNA ISH-positive cells in these five cases was 75.6% (median, 82%; range, 40-94%), whereas the proportion of ALK RNA ISH-positive cells in the remaining 289 cases was 0.3% (median 0%; range, 0-15%). When the cutoff value was set at 15%, ALK RNA ISH-positive and ALK RNA ISH-negative cases were distinguishable with 100% sensitivity and specificity relative to the IHC/ FISH tests. Conclusions Our findings show that RNA ISH is useful for detecting ALK rearrangement with high sensitivity and specificity relative to conventional IHC/ FISH tests. Thus, RNA ISH, which is an easy and rapid assay, could be an alternative method to IHC and FISH.
- Subjects
DIAGNOSTIC use of in-situ hybridization; LUNG cancer diagnosis; RNA; ANAPLASTIC lymphoma kinase; IMMUNOHISTOCHEMISTRY
- Publication
Histopathology, 2017, Vol 71, Issue 1, p143
- ISSN
0309-0167
- Publication type
Article
- DOI
10.1111/his.13198