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- Title
A novel pathway for the production of H<sub>2</sub>S by DAO in rat jejunum.
- Authors
Tang, S.; Huang, D.; An, N.; Chen, D.; Zhao, D.
- Abstract
Background Hydrogen sulfide (H2S) is endogenously generated from L-cysteine (L-Cys) by the enzymes cystathionine- β-synthase ( CBS) and cystathionine- γ-Lyase ( CSE). Hydrogen sulfide is also produced from D-cysteine (D-Cys) by D-Amino acid oxidase ( DAO). Methods The H2S production was measured by the methylene blue assay. The expression of DAO was investigated by Western blotting and immunohistochemistry. The short-circuit current ( Isc) was recorded using the Ussing chamber technique. Key Results The epithelium in rat jejunum possesses DAO, and generates H2S. D-cysteine, originally used as a negative control for L-Cys, significantly increases the H2S release, which is inhibited by I2 CA, an inhibitor of DAO. In vitro study by Ussing chamber technique reveals that D-Cys decreases the Isc across the epithelium of the rat jejunum and enhances the Na+-coupled L-alanine transport. Conclusions & Inferences A novel pathway for the production of H2S by DAO exists in rat jejunum.
- Subjects
ENDOGENOUS hydrogen sulfide; LABORATORY rats; CYSTEINE; AMINO acid oxidase; IMMUNOHISTOCHEMISTRY
- Publication
Neurogastroenterology & Motility, 2016, Vol 28, Issue 5, p687
- ISSN
1350-1925
- Publication type
Article
- DOI
10.1111/nmo.12765