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- Title
Anti--idiotypic activity against anti--myeloperoxidase antibodies in pooled human immunoglobulin.
- Authors
Pall, A.A.; Varagunam, M.; Adu, D.; Smith, N.; Richards, N. T.; Taylor, B.M.; Michael, J.
- Abstract
We investigated the ability of six different pooled human immunoglobulin (PHIG) preparations to inhibit the binding of anti-myeloperoxidase (MPO) antibodies to MPO. All six PHIG preparations inhibited the binding of anti-MPO antibodies from six sera lo MPO in a concentration-dependent manner in the concentration range 0.016-10 mg/ml. There was considerable variation in the ability of each PHIG preparation to inhibit the binding of anti-MPO antibody in a given serum. Further differences were seen in the ability of a given PHIG to inhibit anti-MPO binding in different sera, F(ab')2 fragments from two PHIIG preparations also inhibited in a concentration-dependent manner anti-MPO binding to MPO in all six sera in the concentration range 0.002-2.65 mg/ml, with a maximum inhibition of 42%. Little inhibition was seen with F(ab')2 of normal human IgG from individual donors (1.8-12.2% at the maximum concentration of 2 mg/ml). F(ab')2 fragments from three anti-MPO containing sera and two affinity-purified anti-MPO antibodies were eluted by affinity chromatography from Sepharose-bound PHIG F(ab')2 and showed anti-MPO antibody activity. We have shown that PHIG and F(ab') 2 fragments of PHIG inhibit anti-MPO binding to MPO, and further that F(ab') 2 fragments of PHIG bind to F(ab')2 fragments of anti-MPO antibodies. These observations indicate binding between the variable regions of PHIG and the antigen binding site of anti-MPO antibodies, and are consistent with an anti-idiotypic reaction. The variability seen in the inhibitory effect of the different PHIG preparations in anti-MPO-positive sera implies differences in their anti-idiotype content, while the variability of the inhibitory effect of a particular PHIG preparation between different sera suggests heterogeneity in the idiotypic repertoire of anti-MPO antibodies. Such variations in the inhibitory effect of different PHIG preparations on antibody binding may be an important determinant of their therapeutic effect.
- Subjects
IMMUNOGLOBULINS; BLOOD plasma; CHROMATOGRAPHIC analysis; ANALYTICAL chemistry; GEL permeation chromatography; SERUM
- Publication
Clinical & Experimental Immunology, 1994, Vol 95, Issue 2, p257
- ISSN
0009-9104
- Publication type
Article
- DOI
10.1111/1365-2249.ep15993643