We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Diagnosis of SARS-CoV-2 by RT-PCR Using Different Sample Sources: Review of the Literature.
- Authors
Torretta, Sara; Zuccotti, Gianvincenzo; Cristofaro, Valentina; Ettori, Jacopo; Solimeno, Lorenzo; Battilocchi, Ludovica; D'Onghia, Alessandra; Bonsembiante, Anna; Pignataro, Lorenzo; Marchisio, Paola; Capaccio, Pasquale
- Abstract
Objective: The most widely used diagnostic technique for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection is real-time reverse transcriptase-polymerase chain reaction (RT-PCR). It can be done on different samples: nasopharyngeal swabs (NPS) or oropharyngeal swabs (OPS), and self-collected saliva. However, negative findings do not rule out infection. Methods: A review was conceived to discuss advantages and limitations of the available diagnostic modalities for nonserologic diagnosis of SARS-CoV-2 based on RT-PCR; the article also proposes some practical suggestions to improve diagnostic reliability. Results: A total of 16 papers (corresponding to 452 patients) of the 56 initially identified were included. Most of the papers describe findings from different samples obtained in limited case series; comparative studies are missing. Conclusions: Diagnostic accuracy of NPS and OPS is suboptimal and the risk of contaminated aerosol dispersal is not negligible. The SARS-CoV-2 RNA can be found in self-collected saliva specimens of many infected patients within 7 to 10 days after symptom onset. There is an urgent need for comparative trials to define the diagnostic modality of choice. Adequate education and training of health care personnel is mandatory.
- Subjects
NASOPHARYNX microbiology; SALIVA microbiology; REVERSE transcriptase polymerase chain reaction; PHARYNX; SARS-CoV-2; AEROSOLS; SYSTEMATIC reviews; COVID-19 testing; POLYMERASE chain reaction; ROUTINE diagnostic tests
- Publication
ENT: Ear, Nose & Throat Journal, 2021, Vol 100, p131S
- ISSN
0145-5613
- Publication type
Article
- DOI
10.1177/0145561320953231