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- Title
Petaloid-type cms in carrot is not associated with expression of atp8 ( orfB).
- Authors
Robison, Mary; Wolyn, David
- Abstract
Two different atp8 reading frames with divergent C-terminal extensions were identified in a sterility-inducing mitochondrial type (mitotype) of carrot; examination of three fertility-inducing mitotypes revealed atp8 reading frames that were either terminated at a position conserved among other plant atp8 genes or continued with unique C-terminal extensions. Similarities between the C-terminal extensions suggest ancient common ancestry, but the level of sequence divergence implies lack of functional conservation. Northern analysis indicated that the C-terminal extensions are transcribed and are present within the primary atp8 transcripts. Western analysis revealed that the products of the two extended atp8 reading frames encoded by the sterility-inducing mitotype are significantly smaller than those predicted by complete translation of their C-terminal extensions, but are slightly larger than the products from unextended reading frames. No alterations were observed in atp8 transcripts or proteins isolated from flowers of the sterility-inducing mitotype that were phenotypically fertile as a result of segregation for nuclear fertility-restoring Ms or Rf alleles, and thus, there is no evidence that expression of atp8 is directly involved in the petaloid cms phenotype in carrot.
- Subjects
CARROTS; FERTILITY; GENOTYPE-environment interaction; GENETIC engineering; ADENOSINE triphosphatase; FLORAL morphology
- Publication
Theoretical & Applied Genetics, 2006, Vol 112, Issue 8, p1496
- ISSN
0040-5752
- Publication type
Article
- DOI
10.1007/s00122-006-0253-y