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- Title
First Identification of Culexflavivirus (Flaviviridae) in Brazil.
- Authors
Machado, Daiane Cristina; Mondini, Adriano; Santos Santana, Vinicius dos; Kamiya Yonamine, Patricia Tiemi; Neto, Francisco Chiaravalloti; de Andrade Zanotto, Paolo Marinho; Nogueira, Mauricio Lacerda
- Abstract
Introduction: Culexflavivirus (CxFV) was first isolated in 2007 from Culexpipiens in Japan and then identified in several oth-er countries. Characterization of the CxFV showed that all strains are related to the cell fusing agent virus. In this manu-script we report the first identification of CxFV in South America. Material and Methods: We have collected Culex sp. mosquitoes using BG-Sentinel traps and manual aspira-tors. They were pooled according to genus, species, sex and location. Viral RNA was extracted and multiplex nested PCR was performed to test the presence of Flavivirus. The positive samples were isolated in C6/36 cells and sequenced for phy-logenetic analyses. Results: 265 female Culex mosquitoes pooled in 83 pools were tested with specific CxFV, Saint Lou-is encephalitis virus (SLEV) and West Nile virus (WNV) prim-ers. Our sequence data indicated maximum sequence simi-larity of 97% with CxFV. Discussion: In this study we report the circulation of CxFV in an urban setting where SLEV had previously caused an outbreak. In terms of public health, this is an important finding due to the assumption that the previ-ous exposition of mosquitoes to CxFV might lessen the sus-ceptibility of these mosquitoes to other flaviviruses.
- Subjects
JAPAN; FLAVIVIRUSES; WEST Nile virus; PHYLOGENY; CULEX pipiens; DETECTION of microorganisms
- Publication
Intervirology, 2012, Vol 55, Issue 6, p475
- ISSN
0300-5526
- Publication type
Article
- DOI
10.1159/000337166